A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids

Eckerdt, F.; Alvarez, A.; Bell, J.; Arvanitis, C.; Iqbal, A.; Arslan, A. D.; Hu, B.; Cheng, S. Y.; Goldman, S.; Platanias, L. C.

Biotechniques. 2016 Jan 14; 60(1):43-6


Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)-based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.

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